A simple and effective cryopreservation protocol for the industrially important and model organism, Euglena gracilis

Mahfuzur R Shah; Erin N Morrison; Adam J Noble; Scott C Farrow

doi:10.1016/j.xpro.2021.101043

A simple and effective cryopreservation protocol for the industrially important and model organism, Euglena gracilis

STAR Protoc. 2021 Dec 15;3(1):101043. doi: 10.1016/j.xpro.2021.101043. eCollection 2022 Mar 18.

Authors

Mahfuzur R Shah¹, Erin N Morrison^{1

2}, Adam J Noble¹, Scott C Farrow^{1

2}

Affiliations

¹ Department of Cell Biology, Metabolism and Systems Biology, Noblegen Inc., 2140 East Bank Dr, Peterborough, ON, Canada.
² Environmental and Life Sciences Graduate Program, Trent University, Peterborough, ON, Canada.

Abstract

Euglena gracilis is a source of high-value natural products. A major factor affecting consistent production of Euglena biomass is strain stability. Cryopreservation is a leading strategy for cell-line storage that helps ensure process reproducibility. We developed a simple cryopreservation protocol for heterotrophically cultured Euglena that enables the recovery of cells after 1 year with a cell viability of ≅80%. This protocol is suitable for labs interested in the long-term preservation of heterotrophic cultures of Euglena and related species.

Keywords: Biotechnology and bioengineering; Cell Biology; Microbiology; Model Organisms.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biomass
Cryopreservation
Euglena gracilis*
Euglena*
Reproducibility of Results