Flow cytometry analysis of the subpopulations of mouse keratinocytes and skin immune cells

STAR Protoc. 2021 Dec 16;3(1):101052. doi: 10.1016/j.xpro.2021.101052. eCollection 2022 Mar 18.


Skin is our body's outermost physical barrier and an immunological interface enriched with various immune and non-immune cells. However, efficient generation of single-cell suspensions for flow cytometry analysis can be challenging. Here, we provide protocols to obtain epidermal and whole skin cell suspensions as well as gating strategies to identify mouse keratinocytes and skin immune cell subsets via flow cytometry. For complete details on the use and execution of this protocol, please refer to Sakamoto et al. (2021).

Keywords: Cell isolation; Flow Cytometry/Mass Cytometry; Immunology; Model Organisms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Flow Cytometry / methods
  • Keratinocytes*
  • Mice
  • Skin*
  • Suspensions


  • Suspensions