A continuous T-cell line from a patient with Sézary syndrome

Arch Dermatol Res. 1987;279(5):293-8. doi: 10.1007/BF00431220.

Abstract

A continuous cell line, Se-Ax, from a patient with Sézary syndrome has been established. The Se-Ax cell line is IL-2 dependent, requires human serum for permanent growth, and has the following phenotype: CD1-, CD2+, CD3+, CD4-, CD5-, CD8-, CD20-, CD25+; it expresses the T9, T10, and HLA-DR antigens. This cell line reveals multiple chromosome aberrations as seen in the most abundant abnormal clone in peripheral blood. Therefore, it is not unlikely that it derives from tumor cells. A putative cytotoxic cell line derived from the same patient has only weak killer-cell activity against the autologous permanent cell line: this CD8+ cytotoxic cell line has a 14q+ chromosomal marker. The fact that the patient demonstrated no natural killer-cell or activated killer-cell activity against the Se-Ax cell line may in part explain the successful establishment of the continuous cell line from bulk culture.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Cell Line
  • Chromosome Aberrations
  • Cytotoxicity, Immunologic
  • Female
  • Humans
  • Killer Cells, Natural / immunology
  • Phenotype
  • Sezary Syndrome / genetics
  • Sezary Syndrome / immunology
  • Sezary Syndrome / pathology*
  • T-Lymphocytes / immunology
  • T-Lymphocytes / pathology*