Ex Vivo HIV Infection Model of Cervico-Vaginal and Rectal Tissue

Louise A Ouattara; Nikolas C Vann; Gustavo F Doncel

doi:10.1007/978-1-0716-1871-4_12

Ex Vivo HIV Infection Model of Cervico-Vaginal and Rectal Tissue

Methods Mol Biol. 2022:2407:157-172. doi: 10.1007/978-1-0716-1871-4_12.

Authors

Louise A Ouattara¹, Nikolas C Vann², Gustavo F Doncel²

Affiliations

¹ CONRAD, Eastern Virginia Medical School, Norfolk, VA, USA. yaoal@evms.edu.
² CONRAD, Eastern Virginia Medical School, Norfolk, VA, USA.

PMID: 34985664
DOI: 10.1007/978-1-0716-1871-4_12

Abstract

Globally, the most frequent route of HIV transmission is through sexual intercourse. In women, sexual transmission of HIV involves cervical, vaginal, endometrial, and rectal mucosal exposure to the virus. Here we describe technical protocols for ex vivo cervical, vaginal, and rectal tissue infection models and cultures that can be used to assess tissue susceptibility to infection under different conditions as well as the potential antiviral efficacy of a treatment for HIV prevention or cure.

Keywords: Biopsies; Cervicovaginal; Explants; HIV; HIV endpoints; Immersion model; Polarized model; Rectal.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Cervix Uteri
Female
HIV Infections*
HIV-1*
Humans
Rectum
Vagina

Grants and funding

PEPFAR/PEPFAR/United States