Cell-Free Paper-Based Analysis of Gut Microbiota and Host Biomarkers

Melissa K Takahashi; Xiao Tan; Aaron J Dy

doi:10.1007/978-1-0716-1998-8_22

Cell-Free Paper-Based Analysis of Gut Microbiota and Host Biomarkers

Methods Mol Biol. 2022:2433:351-374. doi: 10.1007/978-1-0716-1998-8_22.

Authors

Melissa K Takahashi^#¹, Xiao Tan^#^{2

3

4

5}, Aaron J Dy^#^{2

5

6}

Affiliations

¹ Department of Biology, California State University Northridge, Northridge, CA, USA. melissa.takahashi@csun.edu.
² Institute for Medical Engineering and Science, Massachusetts Institute of Technology, Cambridge, MA, USA.
³ Division of Gastroenterology, Massachusetts General Hospital, Boston, MA, USA.
⁴ Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, USA.
⁵ Broad Institute of MIT and Harvard, Cambridge, MA, USA.
⁶ Health Advances LLC, Newton, MA, USA.

^# Contributed equally.

PMID: 34985756
DOI: 10.1007/978-1-0716-1998-8_22

Abstract

The gut microbiome and its interactions with the host have been shown to affect several aspects of human health and disease. Investigations to elucidate these mechanisms typically involve sequence analysis of fecal samples. To support these studies, we present methods to design RNA toehold switch sensors to detect microbial and host transcripts. The sensors are embedded in paper-based, cell-free reactions that enable affordable and rapid analysis of microbiome samples.

Keywords: Biosensors; Gut microbiota; Host biomarkers; NASBA; Paper-based diagnostics; RNA toehold switch.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteria / genetics
Biomarkers
Feces
Gastrointestinal Microbiome* / genetics
Humans
RNA, Ribosomal, 16S / genetics

Substances

Biomarkers
RNA, Ribosomal, 16S