Stimulation by bradykinin, angiotensin II, and carbachol of the accumulation of inositol phosphates in PC-12 pheochromocytoma cells: differential effects of lithium ions on inositol mono- and polyphosphates

J Neurochem. 1987 Nov;49(5):1379-85. doi: 10.1111/j.1471-4159.1987.tb01003.x.


Rat PC-12 pheochromocytoma cells respond to stimulation with bradykinin, angiotensin II, and carbachol with an increased formation of labeled inositol phosphates after preincubation of the cells with [3H]inositol. Li+ potentiates greatly the agonist-induced increase in amount of inositol mono-, bis-, and trisphosphate but not the increase in amount of inositol tetrakisphosphate. Separation of the isomers of inositol trisphosphate shows that the lithium-induced increase in amount of inositol trisphosphate is due to potentiation evoked by lithium of the accumulation of inositol-1,3,4-trisphosphate.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenal Gland Neoplasms / metabolism
  • Angiotensin II / pharmacology*
  • Atropine / pharmacology
  • Bradykinin / pharmacology*
  • Carbachol / pharmacology*
  • Cations, Monovalent
  • Cell Line
  • Inositol 1,4,5-Trisphosphate
  • Inositol Phosphates / metabolism*
  • Kinetics
  • Lithium / pharmacology*
  • Pheochromocytoma / metabolism
  • Sugar Phosphates / metabolism*


  • Cations, Monovalent
  • Inositol Phosphates
  • Sugar Phosphates
  • Angiotensin II
  • Atropine
  • Inositol 1,4,5-Trisphosphate
  • Carbachol
  • Lithium
  • Bradykinin