Molecular mechanism of high-production tannase of Aspergillus carbonarius NCUF M8 after ARTP mutagenesis: revealed by RNA-seq and molecular docking

J Sci Food Agric. 2022 Aug 15;102(10):4054-4064. doi: 10.1002/jsfa.11754. Epub 2022 Jan 24.

Abstract

Background: Tannase is an enzyme produced by microbial fermentation and is widely used in the food industry; however, the molecular mechanism of tannase production by Aspergillus has not yet been studied. This study was conducted to reveal the differences in Aspergillus carbonarius tannase enzymatic characterization, secondary structures and molecular mechanisms after treatment of the strain with atmospheric and room temperature plasma (ARTP).

Results: The results showed that the specific activity of tannase was improved by ARTP treatment, and it showed higher thermostability and tolerance to metal ions and additives. The enzymatic characterization and molecular docking results indicated that tannase had a higher affinity and catalytic rate with tannic acid as a substrate after ARTP treatment. In addition, the docking results indicated that Aspergillus tannases may catalyze tannic acid by forming two hydrogen-bonding networks with neighboring residues. RNA-seq analysis indicated that changes in steroid biosynthesis, glutathione metabolism, glycerolipid metabolism, oxidative phosphorylation pathway and mitogen-activated protein kinase signaling pathways might be crucial reasons for the high production of tannase.

Conclusion: ARTP enhanced the yield and properties of A. carbonarius tannase by changing the enzyme structure and cell metabolism. This study provides a theoretical basis for elucidating the molecular mechanism underlying high production of Aspergillus tannases. © 2022 Society of Chemical Industry.

Keywords: Aspergillus carbonarius; enzymatic characterization; molecular docking; tannase; transcriptomics.

MeSH terms

  • Aspergillus* / metabolism
  • Carboxylic Ester Hydrolases* / chemistry
  • Carboxylic Ester Hydrolases* / genetics
  • Molecular Docking Simulation
  • Mutagenesis
  • RNA-Seq
  • Tannins / metabolism

Substances

  • Tannins
  • Carboxylic Ester Hydrolases
  • tannase

Supplementary concepts

  • Aspergillus carbonarius