Paenibacillus albicereus sp. nov. and Niallia alba sp. nov., isolated from digestive syrup

Arch Microbiol. 2022 Jan 8;204(2):127. doi: 10.1007/s00203-021-02749-x.

Abstract

Two aerobic, Gram-stain variable, catalase-positive and oxidase-negative rods named strain UniB2T and UniB3T, were isolated from digestive syrup containing fungal diastase (10 mg/ml), pepsin (2 mg/ml) and sugar base containing polyethylene glycol. Based on 16S rRNA gene sequence analysis, strain UniB2T has the highest sequence similarity with Paenibacillus humicus NBRC 102415T (98.3%) and strain UniB3T showed the highest sequence similarity with Niallia circulans DSM 11T (98.9%). The DNA G + C content of UniB2T was 63.7 mol %. The dDDH and ANI values between the strain UniB2T and its phylogenetically close relative were < 38.3% and < 89.5%, respectively. The major fatty acids of the strain UniB2T were C16:0 (13.9%), C15:0 anteiso (39.7%), C17:0 anteiso (15.5%). The DNA G + C content of UniB3T was 35.6 mol %. The dDDH and ANI values between the strain UniB3T and its close relatives were < 29.1% and 84.6%, respectively. The major fatty acids of strain UniB3T were C16:0 (13.5%), C15:0 anteiso (40.1%) and C17:0 anteiso (16.0%). Major polar lipids for both strains were Diphosphatidylglycerol and phosphatidylethanolamine. Both strains showed unique carbon utilization and assimilation pattern that differentiated them from their phylogenetically related neighbours. These phenotypic, genotypic and chemotaxonomic characters indicated the strains UniB2T and UniB3T represent two novel species for which the names Paenibacillus albicereus sp. nov. (Type strain UniB2T = MCC 3997T = KCTC 43095T = JCM34513T) and Niallia alba sp. nov. (Type strain UniB3T = MCC 3998T = KCTC 43235T = JCM 34492T) are proposed.

Keywords: Bacillus; Fungal diastase; Niallia; Paenibacillus; Pepsin; Polyethylene glycol; α-Amylase.

MeSH terms

  • Bacterial Typing Techniques
  • DNA, Bacterial / genetics
  • Fatty Acids / analysis
  • Paenibacillus* / genetics
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Vitamin K 2

Substances

  • DNA, Bacterial
  • Fatty Acids
  • RNA, Ribosomal, 16S
  • Vitamin K 2