Dual-emission ratiometric fluorescence probe based on copper nanoclusters for the detection of rutin and picric acid

Abstract

In this paper, polyvinylpyrrolidone-templated copper nanoclusters (PVP-CuNCs) were synthesised using a hydrothermal method. Through the electrostatic interaction between PVP-CuNCs and rhodamine 6G, a dual-emission ratiometric fluorescent probe was constructed, and two well-separated emission peaks appeared at 420 nm and 570 nm. The selective detection of rutin and picric acid was achieved by fitting the relationship between the ratiometric fluorescence intensity (F₄₂₀/F₅₇₀) and the concentration of the target detection substance. The limits of detection of rutin and picric acid were 0.84 μM and 0.27 μM, respectively. The synthesised material has high stability and successfully allows the determination of rutin content in drugs and picric acid content in water samples with satisfactory recoveries.

Keywords: Copper nanoclusters; Fluorescent probe; Inner filter effect (IFE); Rhodamine 6G.