Suppression of Wnt Expression by Increasing PI3K in Rats Cervical Carcinoma by Andaliman (Zanthoxylum acanthopodium)

Abstract

&lt;b&gt;Background and Objective:&lt;/b&gt; Cervical cancer is the leading cause of death for women in the world and Indonesia. This disease originates from a malignant tumour of squamous epithelial cells caused by infection with the Human Papilloma Virus (HPV). Antioxidants can reduce oxidative stress in and there are plants from Indonesia that have high antioxidants, namely andaliman (&lt;i&gt;Zanthoxylum acanthopodium&lt;/i&gt;). This study aimed to analyze the role of andaliman on PI3K and Wnt signalling in cervical cancer histology. &lt;b&gt;Materials and Methods:&lt;/b&gt; The study includes 5 treatments. The control group (K-), rats cancer model (K+), rats cancer model+the dose is 100 mg/b.wt. of ZAM (P&lt;sub&gt;1&lt;/sub&gt;), rats cancer model+the dose is 200 mg/b.wt. of ZAM (P&lt;sub&gt;2&lt;/sub&gt;) and rats cancer model+the dosage is 400 mg/b.wt. ZAM (P&lt;sub&gt;3&lt;/sub&gt;). On the 30th day after ZAM administration, the rats were dissected for the paraffin block and Wnt and PI3K immunohistochemical staining was prepared. &lt;b&gt;Results:&lt;/b&gt; There was a significant difference between all groups (p<0.001) in Wnt and PI3K expression. The real role of ZAM in cervical cancer tissue was seen at the highest ZAM dose (P&lt;sub&gt;3&lt;/sub&gt;). Irregular mucosal folds and stretched interstitial connective tissue in the K+ group can return to regularity and improve at the P&lt;sub&gt;3&lt;/sub&gt; dose. The administration of ZAM showed a significant difference in cervical tissue after benzopyrene injection. &lt;b&gt;Conclusion:&lt;/b&gt; Andaliman (&lt;i&gt;Zanthoxylum acanthopodium&lt;/i&gt;) extract increases PI3K expression through suppression of Wnt expression. It can be developed therapy molecularly to prevent cell growth into cancer.

Keywords: Andaliman; PI3K; Wnt; Zanthoxylum acanthopodium; carcinoma cervical; herbal plant; immunohistochemistry.