High-Throughput Single-Cell TCR-pMHC Dissociation Rate Measurements Performed by an Autonomous Microfluidic Cellular Processing Unit

ACS Sens. 2022 Jan 28;7(1):159-165. doi: 10.1021/acssensors.1c01935. Epub 2022 Jan 10.

Abstract

We developed an integrated microfluidic cellular processing unit (mCPU) capable of autonomously isolating single cells and performing measurements and on-the-fly analysis of cell-surface dissociation rates, followed by recovery of selected cells. We performed proof-of-concept, high-throughput single-cell experiments characterizing pMHC-TCR interactions on live CD8+ T cells. The mCPU platform analyzed TCR-pMHC dissociation rates with a throughput of 50 cells per hour and hundreds of cells per run, and we demonstrate that cells can be selected, enriched, and easily recovered from the device.

Keywords: TCR; cancer; immunotherapy; lab-on-chip; microfluidics; pMHC; single-cell analysis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • CD8-Positive T-Lymphocytes
  • Microfluidics*
  • Protein Binding
  • Receptors, Antigen, T-Cell*

Substances

  • Receptors, Antigen, T-Cell