Inhibition of ATP synthesis by 1-methyl-4-phenylpyridinium ion (MPP+) in isolated mitochondria from mouse brains

Y Mizuno; K Suzuki; N Sone; T Saitoh

doi:10.1016/0304-3940(87)90366-1

Inhibition of ATP synthesis by 1-methyl-4-phenylpyridinium ion (MPP+) in isolated mitochondria from mouse brains

Neurosci Lett. 1987 Oct 16;81(1-2):204-8. doi: 10.1016/0304-3940(87)90366-1.

Authors

Y Mizuno¹, K Suzuki, N Sone, T Saitoh

Affiliation

¹ Department of Neurology, Jichi Medical School, Tochigi, Japan.

PMID: 3501080
DOI: 10.1016/0304-3940(87)90366-1

Abstract

Effect of 1-methyl-4-phenylpyridinium ion (MPP+) on adenosine triphosphate (ATP) synthesis was studied using isolated mitochondrial preparations from mouse brains. Oxidation of glutamate + malate in the mitochondria estimated by polarography was significantly inhibited by MPP+, and synthesis of ATP was inhibited to approximately 9% of that of control by 0.06 mM of MPP+. Oxidation of alpha-glycerophosphate and succinate was not inhibited, and ATP was synthesized normally. Energy crisis appears to be one of the most important mechanisms of neuronal degeneration in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced experimental parkinsonism.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

1-Methyl-4-phenylpyridinium
Adenosine Triphosphate / biosynthesis*
Animals
Brain / metabolism*
Kinetics
Male
Mice
Mice, Inbred C57BL
Mitochondria / drug effects
Mitochondria / metabolism*
Neurotoxins / pharmacology*
Pyridinium Compounds / pharmacology*

Substances

Neurotoxins
Pyridinium Compounds
Adenosine Triphosphate
1-Methyl-4-phenylpyridinium