Inhibition of ATP synthesis by 1-methyl-4-phenylpyridinium ion (MPP+) in isolated mitochondria from mouse brains

Neurosci Lett. 1987 Oct 16;81(1-2):204-8. doi: 10.1016/0304-3940(87)90366-1.


Effect of 1-methyl-4-phenylpyridinium ion (MPP+) on adenosine triphosphate (ATP) synthesis was studied using isolated mitochondrial preparations from mouse brains. Oxidation of glutamate + malate in the mitochondria estimated by polarography was significantly inhibited by MPP+, and synthesis of ATP was inhibited to approximately 9% of that of control by 0.06 mM of MPP+. Oxidation of alpha-glycerophosphate and succinate was not inhibited, and ATP was synthesized normally. Energy crisis appears to be one of the most important mechanisms of neuronal degeneration in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced experimental parkinsonism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 1-Methyl-4-phenylpyridinium
  • Adenosine Triphosphate / biosynthesis*
  • Animals
  • Brain / metabolism*
  • Kinetics
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mitochondria / drug effects
  • Mitochondria / metabolism*
  • Neurotoxins / pharmacology*
  • Pyridinium Compounds / pharmacology*


  • Neurotoxins
  • Pyridinium Compounds
  • Adenosine Triphosphate
  • 1-Methyl-4-phenylpyridinium