Effect of Oregano Oil and Cobalt Lactate on Sheep In Vitro Digestibility, Fermentation Characteristics and Rumen Microbial Community

Zhengwen Wang; Xiongxiong Li; Lingyun Zhang; Jianping Wu; Shengguo Zhao; Ting Jiao

doi:10.3390/ani12010118

Effect of Oregano Oil and Cobalt Lactate on Sheep In Vitro Digestibility, Fermentation Characteristics and Rumen Microbial Community

Animals (Basel). 2022 Jan 4;12(1):118. doi: 10.3390/ani12010118.

Authors

Zhengwen Wang¹, Xiongxiong Li^{2

3}, Lingyun Zhang¹, Jianping Wu⁴, Shengguo Zhao^{1

2}, Ting Jiao^{2

3}

Affiliations

¹ College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, China.
² Key Laboratory of Grassland Ecosystem, Gansu Agricultural University, Lanzhou 730070, China.
³ College of Grassland Science, Gansu Agricultural University, Lanzhou 730070, China.
⁴ Gansu Academy of Agricultural Sciences, Lanzhou 730070, China.

Abstract

The objective of this experiment was to evaluate the effect of different EOC (0.1425% cobalt lactate + 1.13% oregano essential oil + 98.7275% carrier) levels on in vitro rumen fermentation and microbial changes. Six EOC levels (treatments: 0 mg·L^-1, CON; 50 mg·L^-1, EOC1; 100 mg·L^-1, EOC2; 400 mg·L^-1, EOC3; 800 mg·L^-1, EOC4 and 1500 mg·L^-1, EOC5) were selected to be used to in vitro incubation. The in vitro dry matter digestibility (IVDMD), in vitro neutral detergent fiber digestibility (IVNDFD), in vitro acid detergent fiber digestibility (IVNDFD), pH, ammonia-nitrogen (NH₃-N) concentration, total volatile fatty acid (TVFA) concentration and microbial protein (MCP) concentration were measured after 48 h incubation, after which the groups with significant nutrient digestibility and fermentation parameters were subjected to 16S rRNA sequencing. The results showed that the total gas production (GP) of the EOC5 group was higher than that of the other groups after 12 h of in vitro incubation. TVFA, NH₃-N and MCP concentrations were also shown to be higher in group EOC5 than those in other groups (p < 0.05), while NH₃-N and MCP concentrations in the EOC2 group were lower than those in other groups significantly (p < 0.05). The molar ratio of acetic acid decreased while the molar ratio of propionic acid increased after the addition of EOC. 16S rRNA sequencing revealed that the rumen microbiota was altered in response to adding EOC, especially for the EOC5 treatment, with firmicutes shown to be the most abundant (43.1%). The relative abundance of Rikenellaceae_RC9_gut_group was significantly lower, while the relative abundance of uncultured_bacterium_f_Muribaculaceae and Succiniclasticum was significantly higher in the EOC5 group than those in other groups (p < 0.05). Comprehensive analysis showed that EOC (1500 mg·L^-1) could significantly increase gas production, alter sheep rumen fermentation parameters and microbiota composition.

Keywords: cobalt lactate; essential oil; fermentation parameters; in vitro; oregano; rumen microorganism.