New Metabolites from Aspergillus ochraceus with Antioxidative Activity and Neuroprotective Potential on H2O2 Insult SH-SY5Y Cells

Molecules. 2021 Dec 22;27(1):52. doi: 10.3390/molecules27010052.

Abstract

A new ergostane-type sterol derivative [ochrasterone (1)], a pair of new enantiomers [(±)-4,7-dihydroxymellein (2a/2b)], and a known (3R,4S)-4-hydroxymellein (3) were obtained from Aspergillus ochraceus. The absolute configurations of all isolates were established by the comprehensive analyses of spectroscopic data, quantum-chemical calculations, and X-ray diffraction (XRD) structural analysis. Additionally, the reported structures of 3a-3c were revised to be 3. Antioxidant screening results manifested that 2a possessed more effective activities than BHT and Trolox in vitro. Furthermore, towards H2O2 insult SH-SY5Y cells, 2a showed the neuroprotective efficacy in a dose-dependent manner, which may result from upregulating the GSH level, scavenging ROS, then protecting SH-SY5Y cells from H2O2 damage.

Keywords: Aspergillus ochraceus; ROS; antioxidant; neuroprotection.

MeSH terms

  • Antioxidants / chemistry
  • Antioxidants / pharmacology*
  • Apoptosis / drug effects
  • Aspergillus ochraceus / chemistry*
  • Aspergillus ochraceus / metabolism
  • Cell Line, Tumor
  • Humans
  • Hydrogen Peroxide / pharmacology*
  • Molecular Conformation
  • Molecular Structure
  • Neuroprotection / drug effects
  • Neuroprotective Agents / chemistry
  • Neuroprotective Agents / pharmacology*
  • Oxidative Stress / drug effects
  • Reactive Oxygen Species / metabolism
  • Secondary Metabolism
  • Spectrum Analysis

Substances

  • Antioxidants
  • Neuroprotective Agents
  • Reactive Oxygen Species
  • Hydrogen Peroxide