Novel mono- and multi-strain probiotics supplementation modulates growth, intestinal microflora composition and haemato-biochemical parameters in broiler chickens

Vet Med Sci. 2022 Mar;8(2):668-680. doi: 10.1002/vms3.709. Epub 2022 Jan 11.


Background: The reduction of antimicrobial usage in food-producing animals necessitates the intense search for novel alternatives, including new probiotic strains with more effective properties in improving growth performance and curtailing diseases in animals.

Objective: This study evaluated the effects of novel mono- and multi-strain probiotics on the growth performance, intestinal microbiota and haemato-biochemical parameters of broilers.

Methods: A total of 160 one-day-old Cobb 500 broilers were divided into eight treatment groups with two replicates consisting of (1) basal diet (negative control), (2) basal diet with antibiotic, colistin sulphate, (3) basal diet with commercial probiotic, PROMAX® (positive control), (4) basal diet with Pediococcus acidilactici I5, (5) basal diet with P. pentosaceus I13, (6) basal diet with Enterococcus faecium C14, (7) basal diet with Lactobacillus plantarum C16 and (8) basal diet with the combination of all the four probiotic strains. Birds were kept for 35 days and through oral gavage, 1 ml of 108 study probiotic strains administered on days 3-6, 14 and 18.

Results: Supplementation with P. pentosaceus I13, L. plantarum C16 or multi-strain probiotics significantly (p < 0.05) improved the body weight gain and feed conversion ratio with decrease in feed intake and intestinal Enterobacteria counts. There was a significant (p < 0.05) increase in haemoglobin, mean corpuscular volume, total white blood cells, platelets counts and a lowered (p < 0.05) total cholesterol and glucose levels in multi-strains probiotic supplemented birds.

Conclusion: The supplementation with novel multi-strain probiotics improved growth, intestinal health and haemato-biochemical parameters in broilers and could be used as suitable antibiotic alternatives.

Keywords: broiler; haemato-biochemical parameter; intestinal microbiota; multi-strain; probiotic.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animal Feed / analysis
  • Animals
  • Anti-Bacterial Agents / pharmacology
  • Chickens
  • Dietary Supplements
  • Gastrointestinal Microbiome*
  • Probiotics* / pharmacology


  • Anti-Bacterial Agents