In this study, carbon dots (CDs) with red color are successfully prepared via hydrothermal treatment of o-phenylenediamine and urea. The as-prepared red CDs exhibit an acidichromism feature, making them turn purple at pH 4.4 and become blue at pH 3.3. Further investigations reveal that the surface chemical bond species of CDs are responsible for the acidichromism feature. Taking advantage of the acidichromism feature, the CDs are employed as a titration indicator for analysis of alkali samples, which gives rise to satisfactory results without significant difference between the titration methods using CDs and methyl orange or a mixture of methyl red and bromocresol green as indicators. The CDs show excitation-independent fluorescence with dual-emission at 600 and 650 nm, along with a respectable quantum yield of 20.1%, which provides the CDs with deep tissue penetration and minimum autofluorescence background that is desirable in bioimaging. In addition, the CDs are found to light up endoplasm reticulum particularly, indicating their endoplasm reticulum targeting capability, which is proven by a colocalization study with other classical subcellular dyes. Endocytosis inhibiting investigations confirm that the endoplasm reticulum targeting ability is mainly attributed to the caveolin/lipid-raft-mediated endocytosis pathways of CDs. This study not only presents a facile approach for red CDs but also explores the possibility of CDs in titration analysis and in endoplasm reticulum targeting imaging.
Keywords: acidichromism feature; acid−base titration; carbon dots; endoplasm reticulum targeting imaging; red fluorescence.