Genome-wide identification and expression analysis of the Hsp gene superfamily in Asian long-horned beetle (Anoplophora glabripennis)

Yabei Xu; Fengming Shi; Yurong Li; Shixiang Zong; Jing Tao

doi:10.1016/j.ijbiomac.2022.01.014

Genome-wide identification and expression analysis of the Hsp gene superfamily in Asian long-horned beetle (Anoplophora glabripennis)

Int J Biol Macromol. 2022 Mar 1:200:583-592. doi: 10.1016/j.ijbiomac.2022.01.014. Epub 2022 Jan 8.

Authors

Yabei Xu¹, Fengming Shi², Yurong Li³, Shixiang Zong⁴, Jing Tao⁵

Affiliations

¹ Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China. Electronic address: xuyabei@bjfu.edu.cn.
² Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China. Electronic address: shifengming@bjfu.edu.cn.
³ Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China. Electronic address: yurong_li@bjfu.edu.cn.
⁴ Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China. Electronic address: zongshixiang@bjfu.edu.cn.
⁵ Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing 100083, China. Electronic address: taojing@bjfu.edu.cn.

PMID: 35016971
DOI: 10.1016/j.ijbiomac.2022.01.014

Abstract

The environmental adaptability of insects has been a key focus of ecological research. As molecular chaperones, Heat shock proteins (Hsps) play an important role in insect responses to environmental stress. Anoplophora glabripennis is a destructive pest of broad-leaved trees such as poplars. The ability to adapt to low temperature is an important factor for successful colonization of A. glabripennis in new diffusion area. However, the roles of Hsp in the stress responses in A. glabripennis have not been established. In this study, we identified 47 Hsp genes, including 3 Hsp90, 14 Hsp70, 9 Hsp60, and 21 sHsp genes. The Hsp gene family expanded substantially in A. glabripennis. The differences in expression patterns may be related to the type and intensity of stress. Larval overwintering transcriptomes showed that 13 Hsp genes were not induced during overwintering and 21 Hsp genes were involved in the regulation of life activities under non-stress conditions. In a quantitative RT-PCR analysis, AglaHsp90-2 responded more quickly under gradient cooling treatments; AglaHsp90-2 and AglaHsp90-3 were sensitive to treatment at 0 °C for 6 h under instantaneous cooling. Our results provide a theoretical basis for clarifying the molecular mechanism of Hsp genes in A. glabripennis in responsing to environmental stresses.

Keywords: Anoplophora glabripennis; Cold stress; Expression profile; Genome-wide analysis; Heat shock protein.

MeSH terms

Animals
Coleoptera*