FACS and immunomagnetic isolation of early erythroid progenitor cells from mouse fetal liver

STAR Protoc. 2021 Dec 29;3(1):101070. doi: 10.1016/j.xpro.2021.101070. eCollection 2022 Mar 18.

Abstract

Early erythroid progenitors are transit-amplifying cells with high proliferative capacity committed to undergoing red cell differentiation. CD71/CD24low progenitors are less mature and have greater proliferative capacity than CD71/CD24high. We present protocols for isolation of CD71/CD24low progenitors from mouse fetal liver using both fluorescence-activated cell sorting (FACS) and immunomagnetic enrichment. CD71/CD24low progenitors isolated with both approaches show similar transcriptomes at single-cell resolution and exhibit characteristic proliferative responses to glucocorticoids. For complete details on the use and execution of this protocol, please refer to Li et al. (2019).

Keywords: Cell Biology; Cell Differentiation; Cell isolation; Developmental biology; Flow Cytometry/Mass Cytometry; Single Cell.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Erythroid Precursor Cells*
  • Erythropoiesis* / physiology
  • Flow Cytometry / methods
  • Liver
  • Mice
  • Transcriptome