Sensitive and real-time detection of IgG using interferometric reflecting imaging sensor system

Biosens Bioelectron. 2022 Apr 1;201:113961. doi: 10.1016/j.bios.2021.113961. Epub 2022 Jan 4.

Abstract

Considering the limitations of well-known traditional detection techniques, innovative research studies have focused on the development of new sensors to offer label-free, highly sensitive, real-time, low-cost, and rapid detection for biomolecular interactions. In this study, we demonstrate immunoglobulin G (IgG) detection in aqueous solutions by using real-time and label-free kinetic measurements of the Interferometric Reflectance Imaging Sensor (IRIS) system. By performing kinetic characterization experiments, the sensor's performance is comprehensively evaluated and a high correlation coefficient value (>0.94) is obtained in the IgG concentration range of 1-50 μg/mL with a low detection limit (0.25 μg/mL or 1.67 nM). Moreover, the highly sensitive imaging system ensures accurate quantification and reliable validation of recorded binding events, offering new perspectives in terms of direct biomarker detection for clinical applications.

Keywords: Immunoglobulin G; Interferometric reflecting imaging sensor; Medical applications; Real-time detection.

MeSH terms

  • Biosensing Techniques*
  • Immunoglobulin G*
  • Interferometry

Substances

  • Immunoglobulin G