Anti-GPVI (HY101) activates platelets in a multicolor flow cytometry panel

Platelets. 2022 Oct 3;33(7):1096-1099. doi: 10.1080/09537104.2022.2027359. Epub 2022 Jan 17.

Abstract

The platelet transmembrane receptor GPVI can be assessed together with other platelet membrane markers in a whole blood multicolor flow cytometry panel. The advantage of combining multiple antibodies in a single tube is the possibility of distinguishing multiple platelet subgroups. In this short communication, we describe an activation problem encountered with anti-GPVI, clone HY101. Activation of platelets was seen after the addition of anti-GPVI in a flow cytometry panel, highlighted by the expression of the activation markers CD62P, PAC-1, CD63, and CD107a. This was also confirmed by platelet aggregation studies.

Keywords: Anti-GPVI; HY101; flow cytometry; platelet.

MeSH terms

  • Blood Platelets* / metabolism
  • Flow Cytometry
  • Humans
  • Platelet Activation
  • Platelet Aggregation
  • Platelet Membrane Glycoproteins* / metabolism

Substances

  • Platelet Membrane Glycoproteins