The preparation of histological specimens from animals and humans is a multi-step process comprising tissue collection, fixation, and dehydration, followed by paraffin embedding. Each process can be achieved using different methods and substances. For example, dehydration may not be required depending on the substance used for embedding. The freezing technique described in the present study can be used for tissue collection and fixation. Tissues obtained using "in vivo cryotechnique (IVCT)" reflect blood flow and protein localization in body fluids at the time of tissue collection, making it an indispensable method in histological analyses of the future. This study utilized the IVCT to capture histological images of dynamic objects from multiple viewpoints and elucidate the mechanism underlying their movement control at the molecular level.
Keywords: 4.1G; Freeze-substitution fixation; In vivo cryotechnique; Schmidt–Lanterman incisure.
© 2022. The Author(s), under exclusive licence to Japanese Association of Anatomists.