Neural labeling and manipulation by neonatal intraventricular viral injection in mice

Mengzhe Wang; Thomas Misgeld; Monika S Brill

doi:10.1016/j.xpro.2021.101081

Neural labeling and manipulation by neonatal intraventricular viral injection in mice

STAR Protoc. 2022 Jan 10;3(1):101081. doi: 10.1016/j.xpro.2021.101081. eCollection 2022 Mar 18.

Authors

Mengzhe Wang¹, Thomas Misgeld^{1

2

3}, Monika S Brill^{1

3}

Affiliations

¹ Institute of Neuronal Cell Biology, Technische Universität München, Biedersteiner Straße 29, 80802 Munich, Germany.
² German Center for Neurodegenerative Diseases (DZNE), Feodor-Lynen-Straße 17, 81377 Munich, Germany.
³ Munich Cluster of Systems Neurology (SyNergy), Feodor-Lynen-Straße 17, 81377 Munich, Germany.

Abstract

This step-by-step protocol provides a fast and easy technique to label and/or genetically manipulate neural cells, achieved by intraventricular injection of viral vectors into neonatal mice under ultrasound guidance. Successful injection of adeno-associated viral vectors (AAV) induces neural transduction as fast as 3 days post injection (dpi) in both the central and peripheral nervous systems. Virally driven expression persists until early adulthood. The same setup enables injection of other viral vectors as well as intramuscular injection. For complete details on the use and execution of this protocol, please refer to Wang et al. (2021) and Brill et al. (2016).

Keywords: Biotechnology and bioengineering; Developmental biology; Gene Expression; Genetics; Microscopy; Model Organisms; Molecular Biology; Neuroscience.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Dependovirus* / genetics
Genetic Vectors*
Injections
Injections, Intraventricular
Mice
Neurons / metabolism