Synaptotagmin 4 and 5 additively contribute to Arabidopsis immunity to Pseudomonas syringae DC3000

Plant Signal Behav. 2022 Dec 31;17(1):2025323. doi: 10.1080/15592324.2021.2025323. Epub 2022 Jan 21.

Abstract

Soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs) are essential for vesicle trafficking in plants. Vesicle-associated membrane protein 721 and 722 (VAMP721/722) are secretory vesicle-localized R-SNAREs, which are involved in a variety of biological processes in plants. Compared to VAMP721/722, a VAMP721/722-interacting plasma membrane (PM)-localized Qa-SNARE is engaged in a rather specific physiological process. This indicates that an in vivo regulator controls an interaction between a Qa-SNARE and VAMP721/722 for a specific cellular activity. We previously reported that synaptotagmin 5 (SYT5) modulates the interaction between SYP132 PM Qa-SNARE and VAMP721/722 for Arabidopsis resistance to Pseudomonas syringae DC3000. In this study, we show that defense against P. syringae DC3000 is compromised in SYT4-lacking plants, which belongs to the same subclade as SYT5. Further elevation of bacterial growth in syt4 syt5-2 plants compared to either syt4 or syt5-2 single mutant suggests that SYT4 and SYT5 play additive roles in Arabidopsis immunity to P. syringae DC3000.

Keywords: P. syringae DC3000; SYT4; SYT5; VAMP721/722; immunity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis Proteins* / genetics
  • Arabidopsis Proteins* / metabolism
  • Arabidopsis* / metabolism
  • Arabidopsis* / microbiology
  • Pseudomonas syringae
  • Qa-SNARE Proteins / genetics
  • Qa-SNARE Proteins / metabolism
  • R-SNARE Proteins / metabolism
  • SNARE Proteins / genetics
  • SNARE Proteins / metabolism
  • Synaptotagmins* / genetics
  • Synaptotagmins* / metabolism

Substances

  • Arabidopsis Proteins
  • Qa-SNARE Proteins
  • R-SNARE Proteins
  • SNARE Proteins
  • SYP132 protein, Arabidopsis
  • VAMP721 protein, Arabidopsis
  • Synaptotagmins

Grants and funding

This work was supported by grants (2021R1A6A3A01086784 to SK and 2021R1F1A1063111 to HSY) from the National Research Foundation, Korea, and a grant (P0016045 to CK) from Korea Institute for Advancement of Technology, Korea.