Nanozymes with multiple activities have drawn immense interest owing to their great prospect in biochemical analysis. Fabricating nanomaterials-based artificial enzymes for multiple-enzyme mimetic activity is a significant challenge. This paper reports a sensitive biosensing platform to mimic the peroxidase, oxidase, and catalase-like activity by bimetallic CuPd embedded holey carbon nitride (CuPd@H-C3N4). Owing to the combination of porous H-C3N4 and bimetallic CuPd nanoparticles, the CuPd@H-C3N4 exhibited a large specific surface area, extremely high mobility and catalytic activity of electrons, resulting in remarkable triple-enzyme mimetic activity. Owing to the excellent oxidase/peroxidase-like activities of CuPd@H-C3N4, a visual colorimetric and ultrasensitive fluorometric biosensing platform was established for the discriminatory detection of glutathione (linear range: 2-40 μM) and glucose (linear range: 0.1-40 μM) in physiological fluids, respectively. The fluorescence detection system showed ultrahigh sensitivity toward H2O2, with a linear range of 30-1500 nM. In addition, a one-step glucose detection strategy was proposed to replace the traditional, complicated two-step detection method, which simplifies the operation steps and improves the detection efficiency. The assay presented in this paper offers an effective multiple-enzymes mimicking detection platform that broaden its promising applications in biomedicine analysis and monitoring.
Keywords: Bimetallic; Carbon nitride; Colorimetric and fluorometric; Triple-enzyme mimetic activity.
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