Revealing the early response of pear (Pyrus bretschneideri Rehd) leaves during Botryosphaeria dothideainfection by transcriptome analysis

Plant Sci. 2022 Feb;315:111146. doi: 10.1016/j.plantsci.2021.111146. Epub 2021 Dec 6.

Abstract

Ring rot disease, which is caused by Botryosphaeria dothidea (B. dothidea), is one of the most serious diseases affecting the pear industry. Currently, knowledge of the mechanism about pear-pathogen interactions is unclear. To explore the early response of pear leaves to B. dothidea infection, we compared the early transcriptome of pear leaves infected with B. dothidea. The results revealed 3248 differentially expressed genes (DEGs) and 4862 DEGs at D2 and D4, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation of DEGs showed that these genes were predominately involved in plant-pathogen interactions, hormone signal transduction and other biosynthesis-related metabolic processes, including glucosinolate accumulation and flavonoid pathway enhancement. However, many hormone- and disease resistance-related genes and transcription factors (TFs) were differentially expressed during B. dothidea infection. These results were consistent with the changes in the physiological characteristics of B. dothidea. In addition, the expression of PbrPUB29, an E3 ubiquitin ligase with a U-box domain, was significantly higher than it was at 0 dpi. PbrPUB29 silencing enhanced the sensitivity of pear leaves to B. dothidea, reflected by more severe symptoms and higher reactive oxygen species (ROS) content in the defective pear seedlings after inoculation, revealing that PbrPUB29 has a significant role in pear disease resistance. In brief, we explored the interaction between pear leaves and B. dothidea at the transcriptome level, implied the early response of pear leaves to pathogens, and identified a hub gene in a B. dothidea-infected pear. These results provide a basis and new strategy for exploring the molecular mechanisms underlying pear-pathogen interactions and disease resistance breeding.

Keywords: Botryosphaeria dothidea; Disease resistance; PbrPUB29; Pear (Pyrus bretschneideri Rehd); RNA-seq; Signal transduction.

MeSH terms

  • Ascomycota / pathogenicity*
  • China
  • Crops, Agricultural / genetics
  • Crops, Agricultural / microbiology
  • Crops, Agricultural / physiology
  • Disease Resistance / genetics*
  • Gene Expression Regulation, Fungal
  • Gene Expression Regulation, Plant
  • Genes, Plant
  • Host-Pathogen Interactions / genetics*
  • Plant Diseases / genetics*
  • Plant Diseases / microbiology
  • Plant Leaves / microbiology*
  • Pyrus / genetics*
  • Pyrus / microbiology*
  • Pyrus / physiology*
  • Transcriptome

Supplementary concepts

  • Botryosphaeria dothidea