A protocol to investigate cellular and circuit mechanisms generating sharp wave ripple oscillations in rodent basolateral amygdala using ex vivo slices

Madhusoothanan B Perumal; Pankaj Sah

doi:10.1016/j.xpro.2021.101085

A protocol to investigate cellular and circuit mechanisms generating sharp wave ripple oscillations in rodent basolateral amygdala using ex vivo slices

STAR Protoc. 2022 Jan 11;3(1):101085. doi: 10.1016/j.xpro.2021.101085. eCollection 2022 Mar 18.

Authors

Madhusoothanan B Perumal¹, Pankaj Sah^{1

2}

Affiliations

¹ Queensland Brain Institute, Brisbane 4072, Australia.
² Joint Center for Neuroscience and Neural Engineering and Department of Biology, Southern University of Science and Technology, Shenzhen, Guangdong Province 518055, China.

Abstract

Basolateral amygdala circuits generate oscillatory network activity to process and remember emotion-tagged events. Ex vivo preparations that recapitulate network activities seen in vivo provide an ideal system to investigate the mechanisms driving these network oscillations. Here we describe an ex vivo preparation of basolateral amygdala slices from rodents for measuring the generated sharp wave ripple oscillations (SWs) using local field potential recording and targeted recording from chandelier neurons that initiate SWs. For complete details on the use and execution of this protocol, please refer to Perumal et al. (2021).

Keywords: Cell Biology; Cognitive Neuroscience; Model Organisms; Neuroscience.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Basolateral Nuclear Complex*
Neurons / physiology
Rodentia