Determining the metabolic impact of postbiotics in mice

Fernando F Anhê; Arshpreet Bhatwa; Jonathan D Schertzer

doi:10.1016/j.xpro.2021.101098

Determining the metabolic impact of postbiotics in mice

STAR Protoc. 2022 Jan 13;3(1):101098. doi: 10.1016/j.xpro.2021.101098. eCollection 2022 Mar 18.

Authors

Fernando F Anhê^{1

2

3}, Arshpreet Bhatwa^{1

2

3}, Jonathan D Schertzer^{1

2

3}

Affiliations

¹ Department of Biochemistry and Biomedical Sciences, McMaster University, 1200 Main St. W., Hamilton, Ontario L8N 3Z5, Canada.
² Farncombe Family Digestive Health Research Institute, McMaster University, 1200 Main St. W., Hamilton, Ontario L8N 3Z5, Canada.
³ Centre for Metabolism, Obesity and Diabetes Research, McMaster University, 1200 Main St. W., Hamilton, Ontario L8N 3Z5, Canada.

Abstract

Postbiotics cooperate to influence immune and metabolic outcomes in the host. Here we describe a protocol for in vivo assessment of blood glucose control following acute administration of lipopolysaccharide (LPS) and peptidoglycan (PGN) in mice. This protocol can be adapted for testing a broad range of microbial molecules and ligands for host immune receptors. Experience with mouse handling is required. For complete details on the use and execution of this protocol, please refer to Anhê et al. (2021) and Cavallari et al. (2017).

Keywords: Health Sciences; Immunology; Metabolism; Microbiology; Model Organisms.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Wall / metabolism
Lipopolysaccharides* / metabolism
Mice
Peptidoglycan* / metabolism

Substances

Lipopolysaccharides
Peptidoglycan

Abstract

Publication types

MeSH terms

Substances

Grants and funding