Human induced pluripotent stem cells generated from epilepsy patients for use as in vitro models for drug screening

Zoe L Hunter; Hannah C Leeson; Mohammed R Shaker; Ernst J Wolvetang; Lata Vadlamudi

doi:10.1016/j.scr.2022.102673

Human induced pluripotent stem cells generated from epilepsy patients for use as in vitro models for drug screening

Stem Cell Res. 2022 Apr:60:102673. doi: 10.1016/j.scr.2022.102673. Epub 2022 Jan 19.

Authors

Zoe L Hunter¹, Hannah C Leeson¹, Mohammed R Shaker¹, Ernst J Wolvetang², Lata Vadlamudi³

Affiliations

¹ The University of Queensland, Australian Institute for Bioengineering & Nanotechnology (AIBN), St. Lucia, Brisbane, QLD 4072, Australia.
² The University of Queensland, Australian Institute for Bioengineering & Nanotechnology (AIBN), St. Lucia, Brisbane, QLD 4072, Australia. Electronic address: e.wolvetang@uq.edu.au.
³ The University of Queensland, UQ Centre for Clinical Research, Herston Brisbane, QLD 4029, Australia; Department of Neurology, Royal Brisbane and Women's Hospital, Herston Brisbane, QLD 4029, Australia.

PMID: 35074713
DOI: 10.1016/j.scr.2022.102673

Abstract

In this paper, we describe the generation and validation of human induced pluripotent stem cell (hiPSC) lines from peripheral blood mononuclear cells (PBMCs) from 6 epilepsy patients using a non-integrative Sendai virus vector. These human cellular models will enable patient-specific drug screening to improve outcomes for individuals with this disorder.

MeSH terms

Cell Differentiation
Cellular Reprogramming
Drug Evaluation, Preclinical
Epilepsy*
Humans
Induced Pluripotent Stem Cells* / metabolism
Leukocytes, Mononuclear
Sendai virus