The silkworm, Bombyx mori, is an important model organism of lepidopteran insects, and its testis is a main male reproductive organ and spermatogenesis place. Studying the testis helps to understand the mechanisms of genetic sterility of lepidopteran insects and to achieve sterile insect technique (SIT) for pest control. Herein, we performed a comparative transcriptome analysis of testes between three biological replicates of the GMS mutant and wild strain 898WB, respectively. In total, 1872 up-regulated genes and 1823 down-regulated genes were identified in the testis of the GMS mutant. Several genes contribute significantly to spermatogenesis and testis development, such as "serine/threonine protein kinase", "organic cation transporter protein", "tyrosine protein kinase", "lncRNAs" and "immune-associated genes". The KEGG pathway analysis shows that the DEGs were annotated to 123 pathways, and 10 pathways were significantly enriched, such as "metabolic pathway", "biosynthesis of amino acids", and "phagosome-lysosome pathway", which are associated with testis development and spermatogenesis. The results of the qPCR expression were consistent with the RNA-seq data, which shows that the RNA-seq results were accurate. The DEGs of the testes between GMS mutant and 898WB were screened by RNA-Seq technology, which provides a reliable reference to understand the molecule mechanism of male sterility of the GMS mutant.
Keywords: Male sterility; Silkworm; Testis; Transcriptome.
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