Remdesivir, Molnupiravir and Nirmatrelvir remain active against SARS-CoV-2 Omicron and other variants of concern

Antiviral Res. 2022 Feb;198:105252. doi: 10.1016/j.antiviral.2022.105252. Epub 2022 Jan 24.

Abstract

We assessed the in vitro antiviral activity of remdesivir and its parent nucleoside GS-441524, molnupiravir and its parent nucleoside EIDD-1931 and the viral protease inhibitor nirmatrelvir against the ancestral SARS-CoV2 strain and the five variants of concern including Omicron. VeroE6-GFP cells were pre-treated overnight with serial dilutions of the compounds before infection. The GFP signal was determined by high-content imaging on day 4 post-infection. All molecules have equipotent antiviral activity against the ancestral virus and the VOCs Alpha, Beta, Gamma, Delta and Omicron. These findings are in line with the observation that the target proteins of these antivirals (respectively the viral RNA dependent RNA polymerase and the viral main protease Mpro) are highly conserved.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine / analogs & derivatives
  • Adenosine / therapeutic use
  • Adenosine Monophosphate / analogs & derivatives*
  • Adenosine Monophosphate / therapeutic use
  • Alanine / analogs & derivatives*
  • Alanine / therapeutic use
  • Animals
  • Antiviral Agents / therapeutic use*
  • COVID-19 Drug Treatment*
  • Cell Line
  • Chlorocebus aethiops
  • Coronavirus 3C Proteases / antagonists & inhibitors
  • Cytidine / analogs & derivatives*
  • Cytidine / therapeutic use
  • Humans
  • Hydroxylamines / therapeutic use*
  • Lactams / therapeutic use*
  • Leucine / therapeutic use*
  • Microbial Sensitivity Tests
  • Nitriles / therapeutic use*
  • Proline / therapeutic use*
  • RNA-Dependent RNA Polymerase / antagonists & inhibitors
  • SARS-CoV-2 / drug effects*
  • Vero Cells
  • Virus Replication / drug effects

Substances

  • Antiviral Agents
  • Hydroxylamines
  • Lactams
  • Nitriles
  • GS-441524
  • remdesivir
  • Adenosine Monophosphate
  • Cytidine
  • nirmatrelvir
  • Proline
  • N(4)-hydroxycytidine
  • RNA-Dependent RNA Polymerase
  • Coronavirus 3C Proteases
  • Leucine
  • Adenosine
  • Alanine
  • molnupiravir

Supplementary concepts

  • SARS-CoV-2 variants