Altered expression of miR-29a-3p and miR-34a-5p by specific inhibition of GSK3β in the MPP+ treated SH-SY5Y Parkinson's model

Morteza Ahmadzadeh-Darinsoo; Mojtaba Ahmadzadeh-Darinsoo; Shahsanam Abbasi; Ehsan Arefian; Claude Bernard; Azita Parvaneh Tafreshi

doi:10.1016/j.ncrna.2021.12.004

Altered expression of miR-29a-3p and miR-34a-5p by specific inhibition of GSK3β in the MPP⁺ treated SH-SY5Y Parkinson's model

Noncoding RNA Res. 2022 Jan 7;7(1):1-6. doi: 10.1016/j.ncrna.2021.12.004. eCollection 2022 Mar.

Authors

Morteza Ahmadzadeh-Darinsoo¹, Mojtaba Ahmadzadeh-Darinsoo¹, Shahsanam Abbasi², Ehsan Arefian³, Claude Bernard⁴, Azita Parvaneh Tafreshi^{1

3}

Affiliations

¹ Department of Medical Biotechnology, National Research Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
² Department of Agricultural Biotechnology, National Research Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
³ School of Biology, College of Science, University of Tehran, Tehran, Iran.
⁴ Australian Regenerative Medicine Institute, Monash University, Melbourne, 3800, Victoria, Australia.

Abstract

In the current study, the effects of 7-BIO as a specific GSK3β inhibitor was examined on cell survival and expression of miR-29a-3p and miR-34a-5p in neurotoxin MPP⁺ treated SH-SY5Y cells. Our findings revealed that while co-treatment of the cells with 7-BIO and MPP⁺ did not alter the toxicity induced by MPP⁺, pretreatment with 3.5 μM 7-BIO for 6 h increased the survival of the 2 mM MPP⁺ treated cells. Also, qRT-PCR analysis of gene expression showed that while miR-29a-3p was unchanged in cells treated with either 2 mM MPP⁺ or 3.5 μM 7-BIO alone, miR-34a-5p was increased by MPP⁺ but decreased by 7-BIO. Pretreatment with 3.5 μM 7-BIO prior to MPP⁺ however, increased miR-29a-3p but decreased miR-34a-5p induced by MPP⁺. We therefore suggest that 7-BIO inhibition of GSK3β alleviates the MPP⁺ induced neurotoxicity by regulating miR-29a-3p and miR-34a-5p expressions in Parkinson's disease model SH-SY5Y cells.

Keywords: 7-BIO; GSK3β inhibitor; MPP+; Neuroprotection; Parkinson's disease; SH-SY5Y; miR-29a; miR-29a-3p; miR-34a; miR-34a-5p.