A rhodamine derivative probe for highly selective detection of Cu(II)

Front Biosci (Landmark Ed). 2022 Jan 18;27(1):28. doi: 10.31083/j.fbl2701028.


Background: Abnormal Cu(II) ions levels may affect many biological functions, and it is of great importance to detect Cu(II) ions in organisms.

Methods: Herein, we report a near-infrared (NIR) fluorescent probe EtRh-N-NH2 for the detection of Cu(II). In the probe structure, a rhodamine core was used, and a hydrazine group was employed as the responsive site. Results & Conlusions: EtRh-N-NH2 displayed sensitive, specific and fast response upon Cu(II) with excellent linear relationship between the concentration and fluorescence emission intensity in 0-1 μM range. The releasing EtRh-COOH exhibited 762 nm of emission wavelength with a 75 nm of Stokes shift.

Keywords: Cu(II) detection; Fluorescent probe; Large Stokes shift.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Fluorescent Dyes* / chemistry
  • Rhodamines / chemistry


  • Fluorescent Dyes
  • Rhodamines