Global DNA methylation changes in treated and untreated MS patients measured over time

J Neuroimmunol. 2022 Mar 15;364:577808. doi: 10.1016/j.jneuroim.2022.577808. Epub 2022 Jan 7.


Multiple sclerosis (MS) is an autoimmune, neurological disease. We investigated genome-wide DNA methylation profiles of CD4+ and CD8+ T cells from MS patients and healthy controls at baseline and a follow-up visit. Patients were all treatment-naïve at baseline, and either on treatment or remained untreated at the follow-up visit. MS patients show more changes in their T cell DNA methylation profiles as compared to healthy controls over time, with the most pronounced differences observed in the untreated MS patients. These findings underline the potential of DNA methylation as biomarkers in MS.

Keywords: CD4(+) T cells; CD8(+) T cells; DNA methylation; Differentially methylated position; Epigenetics; Multiple sclerosis.

MeSH terms

  • Adult
  • CD4-Positive T-Lymphocytes / immunology*
  • CD8-Positive T-Lymphocytes / immunology*
  • DNA Methylation / immunology*
  • Female
  • Humans
  • Immunosuppressive Agents / therapeutic use
  • Middle Aged
  • Multiple Sclerosis, Relapsing-Remitting / drug therapy*
  • Multiple Sclerosis, Relapsing-Remitting / immunology*
  • Transcriptome


  • Immunosuppressive Agents