T lymphocyte population dynamics and function following a primary bovine herpesvirus type-1 infection

Viral Immunol. 1987-1988;1(4):287-304. doi: 10.1089/vim.1987.1.287.

Abstract

Following a primary bovine herpesvirus-1 (BHV-1) infection the concanavalin A (Con A) induced proliferative responses of peripheral blood T lymphocytes were suppressed. This suppression occurred in the absence of detectible serum suppressor factors, suppressor cell activity or decreased accessory cell function. However, regression analysis demonstrated a significant correlation between the percentage of T lymphocytes present within the peripheral blood mononuclear cell (PBMC) population and the amplitude of Con-A-induced lymphocyte proliferative responses (LPR). Direct evidence that a numerical deficit of responder T lymphocytes was limiting LPR was obtained by using an immunomagnetic microsphere (IMM) negative enrichment protocol to produce a PBMC population with a constant percentage (75 +/- 6%) of T lymphocytes. The Con-A-induced LPR of these enriched T lymphocytes remained constant following BHV-1 infection. Flow cytometric (FC) analysis of PBMC indicated that the decreased percentage of circulating T lymphocytes, associated with BHV-1 infection, was caused primarily by a selective depletion of the BoT8 subset. These FC data were consistent with the indirect evidence of increased TH activity, as indicated by elevated Con A-induced IL-2 production. Thus, 2 to 5 days following viral infection, the circulating T lymphocytes were activated as shown by elevated IL-2 production, increased recombinant bovine IL-2 (rBo

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Body Temperature
  • Cattle
  • Cell Separation
  • Concanavalin A / metabolism
  • Flow Cytometry
  • Immunoglobulin Isotypes / analysis
  • Immunosuppression Therapy
  • Infectious Bovine Rhinotracheitis / immunology*
  • Interleukin-2 / analysis
  • Lymphocyte Activation
  • T-Lymphocytes / classification*
  • T-Lymphocytes / immunology
  • Time Factors

Substances

  • Antibodies, Monoclonal
  • Immunoglobulin Isotypes
  • Interleukin-2
  • Concanavalin A