Endothelial Cell Fibrin Gel Angiogenesis Bead Assay

Eva M Clavane; Hannah A Taylor; Richard M Cubbon; Paul J Meakin

doi:10.1007/978-1-0716-2059-5_25

Endothelial Cell Fibrin Gel Angiogenesis Bead Assay

Methods Mol Biol. 2022:2441:321-327. doi: 10.1007/978-1-0716-2059-5_25.

Authors

Eva M Clavane¹, Hannah A Taylor¹, Richard M Cubbon¹, Paul J Meakin²

Affiliations

¹ Discovery and Translational Science Department, Leeds Institute of Cardiovascular and Metabolic Medicine, University of Leeds, Leeds, UK.
² Discovery and Translational Science Department, Leeds Institute of Cardiovascular and Metabolic Medicine, University of Leeds, Leeds, UK. P.J.Meakin@leeds.ac.uk.

PMID: 35099748
DOI: 10.1007/978-1-0716-2059-5_25

Abstract

The fibrin gel angiogenesis bead assay provides a controlled in vitro setting for observing endothelial angiogenic sprouting in response to modified variables. Endothelial cells are coated onto microcarriers and embedded into a fibrin clot containing necessary growth factors. Following a 24-h incubation, endothelial sprouts are imaged using a light microscope. This method is useful for rapidly and affordably investigating the effects of genetic or chemical manipulation to endothelial function.

Keywords: Angiogenesis; Cytodex® 3 beads; Endothelial cells; Fibrin matrix; Sprouting.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biological Assay
Endothelial Cells*
Fibrin*
Neovascularization, Physiologic / physiology

Substances

Fibrin

Abstract

Publication types

MeSH terms

Substances

Grants and funding