Isolation of two genes that affect mitotic chromosome transmission in S. cerevisiae

Cell. 1986 Jan 17;44(1):53-63. doi: 10.1016/0092-8674(86)90484-8.


Two DNA sequences that reduce mitotic fidelity of chromosome transmission have been identified: MIF1 and MIF2. MIF1 is a unique sequence located on the right arm of chromosome XII that stimulates loss and recombination for both chromosomes V and VII when present in a high copy number plasmid. MIF1 is not essential for cell division but is necessary for the normal fidelity of chromosome transmission. MIF2 is a unique sequence located 15 cM distal to HIS6 on chromosome IX that induces a high frequency of chromosome VII loss and a lower frequency of chromosome V loss when present in high copy number; it has no effect on mitotic recombination. Disruption of the genomic MIF2 locus was lethal and cells lacking this function arrested division with a terminal phenotype characteristic of a block in DNA replication or nuclear division.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Aneuploidy
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA, Fungal / genetics
  • Gene Expression Regulation
  • Genes, Fungal*
  • Mitosis*
  • RNA, Fungal / analysis
  • Recombination, Genetic
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / physiology


  • DNA, Fungal
  • RNA, Fungal