Mitf is required for T cell maturation by regulating dendritic cell homing to the thymus

Biochem Biophys Res Commun. 2022 Mar 12:596:29-35. doi: 10.1016/j.bbrc.2022.01.091. Epub 2022 Jan 26.


Thymic dendritic cells (DCs) promote immune tolerance by regulating negative selection of autoreactive T cells in the thymus. How DC homing to the thymus is transcriptionally regulated is still unclear. Microphthalmia-associated transcription factor (Mitf) is broadly expressed and plays essential roles in the hematopoietic system. Here, we used Mitf-mutated mice (Mitfvit/vit) and found enlargement of the thymus and expansion of CD4/CD8 double-positive T cells. Mitf was highly expressed in a subset of thymic DCs among the hematopoietic system. Genetic mutation or pharmacological inhibition of Mitf in DCs decreased the expression levels of Itga4, which are critical molecules for the homing of DCs to the thymus. Further, inhibition of Mitf decreased thymic DC number. These results suggest a pivotal role of Mitf in the maintenance of T cell differentiation by regulating the homing of DC subsets within the thymus.

Keywords: Mitf; T cell differentiation; Thymic dendritic cell.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bone Marrow Cells / immunology
  • Bone Marrow Cells / metabolism
  • Cell Differentiation / genetics
  • Cell Differentiation / immunology*
  • Cells, Cultured
  • Dendritic Cells / immunology*
  • Dendritic Cells / metabolism
  • Flow Cytometry
  • Gene Expression Regulation / immunology
  • Hyperplasia
  • Integrin alpha4 / genetics
  • Integrin alpha4 / immunology
  • Integrin alpha4 / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Microphthalmia-Associated Transcription Factor / genetics
  • Microphthalmia-Associated Transcription Factor / immunology*
  • Microphthalmia-Associated Transcription Factor / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • T-Lymphocytes / immunology*
  • T-Lymphocytes / metabolism
  • Thymus Gland / immunology*
  • Thymus Gland / metabolism
  • Thymus Gland / pathology


  • Microphthalmia-Associated Transcription Factor
  • Mitf protein, mouse
  • Integrin alpha4