Zinc finger protein Zfp335 controls early T-cell development and survival through β-selection-dependent and -independent mechanisms

Elife. 2022 Feb 3:11:e75508. doi: 10.7554/eLife.75508.


T-cell development in the thymus undergoes the process of differentiation, selective proliferation, and survival from CD4-CD8- double negative (DN) stage to CD4+CD8+ double positive (DP) stage prior to the formation of CD4+ helper and CD8+ cytolytic T cells ready for circulation. Each developmental stage is tightly regulated by sequentially operating molecular networks, of which only limited numbers of transcription regulators have been deciphered. Here, we identified Zfp335 transcription factor as a new player in the regulatory network controlling thymocyte development in mice. We demonstrate that Zfp335 intrinsically controls DN to DP transition, as T-cell-specific deficiency in Zfp335 leads to a substantial accumulation of DN3 along with reduction of DP, CD4+, and CD8+ thymocytes. This developmental blockade at DN stage results from the impaired intracellular TCRβ (iTCRβ) expression as well as increased susceptibility to apoptosis in thymocytes. Transcriptomic and ChIP-seq analyses revealed a direct regulation of transcription factors Bcl6 and Rorc by Zfp335. Importantly, enhanced expression of TCRβ and Bcl6/Rorc restores the developmental defect during DN3 to DN4 transition and improves thymocytes survival, respectively. These findings identify a critical role of Zfp335 in controlling T-cell development by maintaining iTCRβ expression-mediated β-selection and independently activating cell survival signaling.

Keywords: T-cell development; apoptosis; cell biology; immunology; inflammation; mouse; thymocyte; β-selection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • CD4 Antigens / metabolism
  • CD8 Antigens / metabolism
  • Cell Differentiation
  • Cell Survival*
  • Mice
  • Proto-Oncogene Proteins c-bcl-6 / metabolism
  • Receptors, Antigen, T-Cell, alpha-beta / metabolism*
  • T-Lymphocytes / metabolism*
  • Thymocytes / metabolism
  • Thymus Gland / metabolism
  • Transcription Factors / metabolism*
  • Zinc Fingers


  • CD4 Antigens
  • CD8 Antigens
  • Proto-Oncogene Proteins c-bcl-6
  • Receptors, Antigen, T-Cell, alpha-beta
  • Transcription Factors
  • zinc finger protein 335, mouse

Associated data

  • GEO/GSE184532
  • GEO/GSE184705

Grants and funding

The funders had no role in study design, data collection, and interpretation, or the decision to submit the work for publication.