Phagocytosis by an HIV antibody is associated with reduced viremia irrespective of enhanced complement lysis

Nat Commun. 2022 Feb 3;13(1):662. doi: 10.1038/s41467-022-28250-7.


Increasingly, antibodies are being used to treat and prevent viral infections. In the context of HIV, efficacy is primarily attributed to dose-dependent neutralization potency and to a lesser extent Fc-mediated effector functions. It remains unclear whether augmenting effector functions of broadly neutralizing antibodies (bNAbs) may improve their clinical potential. Here, we use bNAb 10E8v4 targeting the membrane external proximal region (MPER) to examine the role of antibody-mediated effector and complement (C') activity when administered prophylactically against SHIV challenge in rhesus macaques. With sub-protective dosing, we find a 78-88% reduction in post-acute viremia that is associated with 10E8v4-mediated phagocytosis acting at the time of challenge. Neither plasma nor tissue viremic outcomes in vivo is improved with an Fc-modified variant of 10E8v4 enhanced for C' functions as determined in vitro. These results suggest that effector functions inherent to unmodified 10E8v4 contribute to efficacy against SHIVSF162P3 in the absence of plasma neutralizing titers, while C' functions are dispensable in this setting, informing design of bNAb modifications for improving protective efficacy.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Antibody-Dependent Cell Cytotoxicity / drug effects
  • Antibody-Dependent Cell Cytotoxicity / immunology
  • Broadly Neutralizing Antibodies / immunology*
  • Broadly Neutralizing Antibodies / metabolism
  • Broadly Neutralizing Antibodies / pharmacology
  • Cell Line, Tumor
  • Complement System Proteins / immunology*
  • Complement System Proteins / metabolism
  • Cytokines / immunology
  • Cytokines / metabolism
  • Female
  • HIV Antibodies / immunology*
  • HIV Antibodies / metabolism
  • HIV Antibodies / pharmacology
  • HIV Infections / immunology
  • HIV Infections / prevention & control
  • HIV Infections / virology
  • HIV-1 / drug effects
  • HIV-1 / immunology*
  • HIV-1 / physiology
  • Humans
  • Leukocytes, Mononuclear / drug effects
  • Leukocytes, Mononuclear / immunology
  • Leukocytes, Mononuclear / metabolism
  • Macaca mulatta
  • Male
  • Phagocytosis / drug effects
  • Phagocytosis / immunology*
  • Simian Acquired Immunodeficiency Syndrome / immunology
  • Simian Acquired Immunodeficiency Syndrome / prevention & control
  • Simian Acquired Immunodeficiency Syndrome / virology
  • Simian Immunodeficiency Virus / drug effects
  • Simian Immunodeficiency Virus / immunology
  • Simian Immunodeficiency Virus / physiology
  • Viremia / blood
  • Viremia / immunology*
  • Viremia / prevention & control


  • Broadly Neutralizing Antibodies
  • Cytokines
  • HIV Antibodies
  • Complement System Proteins