lncRNA SNHG26 promoted the growth, metastasis, and cisplatin resistance of tongue squamous cell carcinoma through PGK1/Akt/mTOR signal pathway

Qingkun Jiang; Zhonghao Wang; Qi Qi; Jialun Li; Yuqi Xin; Jiaxuan Qiu

doi:10.1016/j.omto.2021.12.021

lncRNA SNHG26 promoted the growth, metastasis, and cisplatin resistance of tongue squamous cell carcinoma through PGK1/Akt/mTOR signal pathway

Mol Ther Oncolytics. 2021 Dec 31:24:355-370. doi: 10.1016/j.omto.2021.12.021. eCollection 2022 Mar 17.

Authors

Qingkun Jiang¹, Zhonghao Wang¹, Qi Qi², Jialun Li¹, Yuqi Xin¹, Jiaxuan Qiu¹

Affiliations

¹ Department of Oral and Maxillofacial Surgery, First Affiliated Hospital of Nanchang University, 17 Yongwai Zheng Street, Nanchang, Jiangxi 330006, China.
² Department of Ultrasound, First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi 330006, China.

Abstract

Tongue squamous cell carcinoma (TSCC) is closely linked to head and neck cancers. Here, we sought to explore the role and mechanism of lncRNAs in the occurrence and progression of TSCC and cisplatin resistance. The results of next-generation transcriptomic sequencing revealed that lncRNA-SNHG26 was differentially expressed and was associated with TSCC cisplatin resistance. The Cancer Genome Atlas dataset and tumor tissue analysis revealed that high SHNG26 expression was associated with the occurrence, progression, and poor prognosis of TSCC. Evidence from cell and animal experiments showed that SNHG26 expression was positively correlated with TSCC proliferation, epithelial-mesenchymal transformation, migration, invasion, and cisplatin resistance. Furthermore, in TSCC cells, SNHG26 was found to bind directly to the PGK1 protein, inhibiting its ubiquitination and activating the Akt/mTOR signaling pathway. These findings suggest that lncRNA-SNHG26 may be a promising target for inhibiting TSCC progression and improving sensitivity to cisplatin chemotherapy in TSCC.

Keywords: Akt/mTOR; PGK1; chemotherapy resistance; cisplatin; epithelial-mesenchymal transformation; lncRNA-SNHG26; ubiquitination; ubiquitination.Tongue squamous cell carcinoma.