Genes for cytochrome P-450 and their regulation

CRC Crit Rev Biochem. 1986;19(3):247-305. doi: 10.3109/10409238609084657.

Abstract

The capacity of the liver microsomal mixed-function oxidase system to metabolize a wide variety of exogenous as well as endogenous compounds reflects the participation of multiple forms of the terminal oxidase, cytochrome P-450, which have different broad, but overlapping, substrate specificities. Several of these isozymes accumulate in the liver after exposure of animals to specific inducing agents. Recent studies employing recombinant DNA techniques to investigate the genetic and evolutionary relatedness of various cytochrome P-450 isozymes as well as the molecular basis for the induction phenomenon are described. The conclusions from these investigations are presented in the context of the substantial body of data obtained from the characterization of specific cytochrome P-450 isozymes and from studies on the induction of specific isozymes or enzymatic activities during development or after treatment of animals with various inducing agents.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Cytochrome P-450 Enzyme System / biosynthesis
  • Cytochrome P-450 Enzyme System / genetics*
  • DNA, Recombinant / metabolism
  • Genes* / drug effects
  • Genes, Regulator* / drug effects
  • Isoenzymes / genetics
  • Microsomes, Liver / drug effects
  • Microsomes, Liver / metabolism
  • Phenobarbital / pharmacology
  • RNA, Messenger / genetics
  • Sequence Homology, Nucleic Acid

Substances

  • DNA, Recombinant
  • Isoenzymes
  • RNA, Messenger
  • Cytochrome P-450 Enzyme System
  • Phenobarbital