Biomolecular alterations in the early life stages of four food fish following acute exposure of Triclosan

Owias Iqbal Dar; Raouf Aslam; Sunil Sharma; Ai-Qun Jia; Arvinder Kaur; Caterina Faggio

doi:10.1016/j.etap.2022.103820

Biomolecular alterations in the early life stages of four food fish following acute exposure of Triclosan

Environ Toxicol Pharmacol. 2022 Apr:91:103820. doi: 10.1016/j.etap.2022.103820. Epub 2022 Feb 2.

Authors

Owias Iqbal Dar¹, Raouf Aslam², Sunil Sharma³, Ai-Qun Jia⁴, Arvinder Kaur⁵, Caterina Faggio⁶

Affiliations

¹ Aquatic Toxicology Lab, Department of Zoology, Guru Nanak Dev University, Amritsar, Punjab 143005 India. Electronic address: owiaszolo@gmail.com.
² Department of Processing and Food Engineering, Punjab Agricultural University, Ludhiana, Punjab 141004, India.
³ Aquatic Toxicology Lab, Department of Zoology, Guru Nanak Dev University, Amritsar, Punjab 143005 India.
⁴ School of Pharmaceutical Sciences, State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou 570228, China.
⁵ Aquatic Toxicology Lab, Department of Zoology, Guru Nanak Dev University, Amritsar, Punjab 143005 India. Electronic address: arvinder.zoology@gndu.ac.in.
⁶ Department of Chemical, Biological, Pharmaceutical and Environmental Sciences University of Messina, Italy. Electronic address: caterina.faggio@unime.it.

PMID: 35123018
DOI: 10.1016/j.etap.2022.103820

Abstract

We investigated the effect of acute concentrations of triclosan (TCS; 96 h exposure and 10d post exposure) on the free amino acid, primary (SDS-PAGE) and secondary (FT-IR) structure of proteins in the embryos/larvae of Cyprinus carpio, Ctenopharyngodon idella, Labeo rohita and Cirrhinus mrigala. A concentration dependent increase in free amino acids, upregulation of polypeptides (100 and 70 kDa in C. carpio, C. idella and L. rohita, 55, 45, 36 kda in C. idella and L. rohita and 22 kDa in all the fish) and a decline in percent area of all the selected peaks of the FT-IR spectra was observed after exposure and recovery period. The decline in percent area was greatest for L. rohita at peak 1080 - 1088 cm^-1 (-75.99%) after exposure and at peak 2854 - 2855 cm^-1 (-53.59%) after recovery. Curve fitting analysis revealed a decrease in α-helices and increase in β-sheets in all fish after exposure and recovery period. The results suggest that TCS elicits alterations in biomolecules of fish embryos.

Keywords: Early life stages; Food fish; Proteomics; Triclosan.

MeSH terms

Animals
Carps* / metabolism
Cyprinidae* / metabolism
Spectroscopy, Fourier Transform Infrared
Triclosan* / toxicity
Water Pollutants, Chemical* / metabolism
Water Pollutants, Chemical* / toxicity

Substances

Water Pollutants, Chemical
Triclosan