Enzyme-linked immunosorbent assay for detection of Clostridium botulinum toxin type A

Jpn J Med Sci Biol. 1978 Feb;31(1):81-5. doi: 10.7883/yoken1952.31.81.


The enzyme linked immunosorbent assay using the so-called "double-sandwich technique" has been applied to determine botulinum toxin type A. By this assay, 50-100 mouse ip LD50 of toxin type A can be detected. No cross-reaction occurs with botulinum toxins of other types tested. In all probability this is due to the high specificity of the antiserum prepared against the toxic component of type A toxin.

MeSH terms

  • Animals
  • Botulinum Toxins / analysis*
  • Botulinum Toxins / immunology
  • Clostridium botulinum / metabolism*
  • Enzyme-Linked Immunosorbent Assay*
  • Immunoenzyme Techniques*
  • Lethal Dose 50
  • Rabbits


  • Botulinum Toxins