Molecular detection of polycystic kidney disease in Persian and Persian-related breeds in Iran

Ali Moazezi Ghavihelm; Shahram Jamshidi; Iraj Ashrafi Tamai; Mahsa Zangisheh

doi:10.1177/20551169211070991

Molecular detection of polycystic kidney disease in Persian and Persian-related breeds in Iran

JFMS Open Rep. 2022 Jan 30;8(1):20551169211070991. doi: 10.1177/20551169211070991. eCollection 2022 Jan-Jun.

Authors

Ali Moazezi Ghavihelm¹, Shahram Jamshidi¹, Iraj Ashrafi Tamai², Mahsa Zangisheh³

Affiliations

¹ Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
² Department of Microbiology, College of Veterinary Medicine, University of Tehran, Tehran, Iran.
³ Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.

Abstract

Objectives: This study was aimed at detecting feline autosomal dominant polycystic kidney disease in a population of Persian and Persian-related breeds by a molecular method in Iran.

Methods: Buccal swab samples were collected from 47 cats and examined with a touchdown PCR method. Additionally, partial sequencing was performed in two cats with bilateral renal cysts.

Results: Twenty-two cats (46.8%) were diagnosed as heterozygous for this mutation. Sequence analysis of two cats showed C to A point mutation in the PKD1 gene, as in previous studies.

Conclusions and relevance: Prevalence of this disease is high in Iran, highlighting the need for molecular screening tests before including cats in breeding programmes.

Keywords: Iran; Persian cats; Polycystic kidney disease; touchdown PCR.