The 14-3-3 protein GF14c positively regulates immunity by modulating the protein homoeostasis of the GRAS protein OsSCL7 in rice

Plant Cell Environ. 2022 Apr;45(4):1065-1081. doi: 10.1111/pce.14278. Epub 2022 Feb 17.

Abstract

Various types of transcription factors have been reported to be involved in plant-pathogen interactions by regulating defence-related genes. GRAS proteins, plant- specific transcription factors, have been shown to play essential roles in plant growth, development and stress responses. By performing a transcriptome study on rice early defence responses to Magnaporthe oryzae, we identified a GRAS protein, OsSCL7, which was induced by M. oryzae infection. We characterized the function of OsSCL7 in rice disease resistance. OsSCL7 was upregulated upon exposure to M. oryzae and pathogen-associated molecular pattern treatments, and knocking out OsSCL7 resulted in decreased disease resistance of rice to M. oryzae. In contrast, overexpression of OsSCL7 could improve rice disease resistance to M. oryzae. OsSCL7 was mainly localized in the nucleus and showed transcriptional activity. OsSCL7 can interact with GF14c, a 14-3-3 protein, and loss-of-function GF14c leads to enhanced susceptibility to M. oryzae. Additionally, OsSCL7 protein levels were reduced in the gf14c mutant and knocking out OsSCL7 affected the expression of a series of defence-related genes. Taken together, these findings uncover the important roles of OsSCL7 and GF14c in plant immunity and a potential mechanism by which plants fine-tune immunity by regulating the protein stability of a GRAS protein via a 14-3-3 protein.

Keywords: 14-3-3; GF14c; GRAS; M. oryzae; OsSCL7; disease resistance; rice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 14-3-3 Proteins / genetics
  • 14-3-3 Proteins / metabolism
  • Disease Resistance / genetics
  • Gene Expression Regulation, Plant
  • Magnaporthe* / metabolism
  • Oryza* / metabolism
  • Plant Diseases
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Proteostasis
  • Transcription Factors / genetics
  • Transcription Factors / metabolism

Substances

  • 14-3-3 Proteins
  • Plant Proteins
  • Transcription Factors