Activation of meiosis and sporulation by repression of the RME1 product in yeast

Nature. 1986 Feb 27-Mar 5;319(6056):738-42. doi: 10.1038/319738a0.


The ability of the yeast Saccharomyces cerevisiae to enter meiosis and sporulate is conferred by the a1-alpha 2 regulatory activity of the mating-type locus. We have cloned the RME1 gene and show that: the RME1 transcript is repressed by a1-alpha 2 in a/alpha cells; overexpression of the RME1 product in a/alpha cells blocks sporulation; and inactivation of RME1 by a null mutation constructed in vitro permits cells lacking a1-alpha 2 activity to sporulate. The RME1 product thus inhibits meiosis; the mating-type locus permits entry into meiosis by repression of RME1.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Chromosome Mapping
  • DNA, Fungal / analysis
  • Gene Expression Regulation
  • Meiosis*
  • Saccharomyces cerevisiae / cytology*
  • Spores, Fungal
  • Transcription, Genetic


  • DNA, Fungal