The effect of micronutrient supplementation on serum anti-Mullerian hormone levels: a retrospective pilot study

Gynecol Endocrinol. 2022 Apr;38(4):310-313. doi: 10.1080/09513590.2022.2028770. Epub 2022 Feb 11.


Background: The anti-Müllerian-hormone (AMH) is secreted by the granulosa cells of the oocytes and can be used as a marker of the ovarian reserve; helpful to estimate female fertility or the menopause onset. Although various factors may influence AMH levels, the correlation with nutritional factors needs more research.

Objective: To evaluate the effect of a micronutrient supplementation on female AMH levels.

Methods: This retrospective analysis includes a total of 244 women, who attended the Karl Landsteiner Institute, Korneuburg, Austria from January 2013 to June 2019 due to an unfulfilled desire for a child. All women were treated with an oral micronutrient preparation consisting the dosage of one soft capsule and one tablet per day for 3 months. The soft capsule contains omega-3 fatty acids and the tablet is a standardized combination of coenzyme Q10, vitamin E, folic acid, selenium, catechins from green tea extract, and glycyrrhizin from licorice extract. Serum AMH levels before and after 3 months were compared. In addition, available clinical data such as ovulation frequency, endometrium thickness, and luteal phase duration were analyzed.

Results: The mean age of the women was 37.3 ± 1.8 years, the mean body mass index of 24.3 ± 4.6 k/m2. The mean serum AMH levels and endometrial thickness values were significantly higher after micronutrient supplementation as compared to baseline (1.42 ± 0.86 versus 1.86 ± 0.82 ng/mL and 6.10 ± 1.76 versus 7.29 ± 1.65 mm, respectively). In addition, ovulation frequency and luteal phase duration significantly improved in more than 60%.

Conclusion: Proposed micronutrient supplementation had a positive effect on serum AMH levels, endometrial thickness, ovulation frequency, and luteal phase duration. It could be a simple, risk-free therapeutic option to improve female fertility. More research is warranted to prove this effect.

Keywords: Infertility; anti-Müllerian-hormone; fertility; micronutrients; supplementation.

MeSH terms

  • Adult
  • Anti-Mullerian Hormone*
  • Dietary Supplements
  • Female
  • Humans
  • Micronutrients*
  • Pilot Projects
  • Retrospective Studies


  • Micronutrients
  • Anti-Mullerian Hormone