Isolation and identification of oral Candida species in potentially malignant disorder and oral squamous cell carcinoma

Chandra Prakash Arya; Rohit Jaiswal; Aanchal Tandon; Amol Jain

doi:10.4103/njms.NJMS_80_19

Isolation and identification of oral Candida species in potentially malignant disorder and oral squamous cell carcinoma

Natl J Maxillofac Surg. 2021 Sep-Dec;12(3):387-391. doi: 10.4103/njms.NJMS_80_19. Epub 2021 Dec 13.

Authors

Chandra Prakash Arya¹, Rohit Jaiswal¹, Aanchal Tandon¹, Amol Jain¹

Affiliation

¹ Department of Oral Pathology and Microbiology, Sardar Patel Postgraduate Institute of Dental and Medical Sciences, Lucknow, Uttar Pradesh, India.

Abstract

Context: Oral cancer is one of the ten most common cancers in the world. More than 95% of the carcinomas of the oral cavity are of squamous cell type in nature. Oral Candida is a "yeast-like opportunistic pathogen." The Candida genus is comprised of over 150 species of asporogenous "yeast-like" fungi.

Aim and objectives: The aim of study is to correlate the association of oral fungal infection in progression of oral squamous cell carcinoma (OSCC) and potentially malignant disorders. The current study was undertaken to probe the isolation and identification of oral Candida species in potentially malignant disorder and OSCC versus normal oral mucosa.

Materials and methods: Twenty patients for each abovementioned three lesions were randomly selected by using swabs. These swabs were subsequently inoculated in agar medium. Candida grows as white, convex colonies. Samples growing 1-3 colony-forming units (CFUs) were considered normal flora of the oral cavity. The specimens showing moderate to heavy growth were subjected to tests for identification of species of Candida. The chromogenic medium, HiMedia CHROMagar, has chromogenic substances which helps in the quick detection of Candida species, based on the reactions between the extract enzymes of the dissimilar species and the chromogenic substances.

Statistical analysis: Chi-square test, one-way analysis of variance test, and post hoc Tukey's test were utilized.

Results: According to our study, Candida albicans, Candida krusei, Candida tropicalis, and Candida parapsilosis in the culture were found to be in increasing incidence from healthy, OSCC, and oral potentially malignant disorders (OPMDs). These results clearly indicated that Candida species are increasing in the CFUs (P < 0.0001).

Conclusion: Our study showed a higher intensity of Candida in OPMD and squamous cell carcinoma patients with results. The increasing CFU level and hyphae of Candida species in individual biopsy tissue with oral potentially malignant lesions to OSCC suggest that this pathogen plays a role in disease development and could aid in identifying the pathogenic commensal.

Keywords: CHROMagar; Candida; Sabouraud dextrose agar; oral squamous cell carcinoma; potentially malignant disorders.