Magnesium Homeostasis in Myogenic Differentiation-A Focus on the Regulation of TRPM7, MagT1 and SLC41A1 Transporters

Int J Mol Sci. 2022 Jan 31;23(3):1658. doi: 10.3390/ijms23031658.


Magnesium (Mg) is essential for skeletal muscle health, but little is known about the modulation of Mg and its transporters in myogenic differentiation. Here, we show in C2C12 murine myoblasts that Mg concentration fluctuates during their differentiation to myotubes, declining early in the process and reverting to basal levels once the cells are differentiated. The level of the Mg transporter MagT1 decreases at early time points and is restored at the end of the process, suggesting a possible role in the regulation of intracellular Mg concentration. In contrast, TRPM7 is rapidly downregulated and remains undetectable in myotubes. The reduced amounts of TRPM7 and MagT1 are due to autophagy, one of the proteolytic systems activated during myogenesis and essential for the membrane fusion process. Moreover, we investigated the levels of SLC41A1, which increase once cells are differentiated, mainly through transcriptional regulation. In conclusion, myogenesis is associated with alterations of Mg homeostasis finely tuned through the modulation of MagT1, TRPM7 and SLC41A1.

Keywords: C2C12 cells; MagT1; SLC41A1; TRPM7; autophagy; magnesium; myogenesis.

MeSH terms

  • Animals
  • Cation Transport Proteins / genetics
  • Cation Transport Proteins / metabolism*
  • Cell Differentiation*
  • Cell Line
  • Magnesium / metabolism*
  • Mice
  • Muscle Development*
  • Myoblasts / metabolism*
  • TRPM Cation Channels / genetics
  • TRPM Cation Channels / metabolism*


  • Cation Transport Proteins
  • Slc41a1 protein, mouse
  • TRPM Cation Channels
  • Trpm7 protein, mouse
  • Magnesium