Isolation and localization of filamin in heart muscle

Eur J Biochem. 1986 May 2;156(3):619-23. doi: 10.1111/j.1432-1033.1986.tb09622.x.

Abstract

High-molecular-mass protein was isolated from chicken heart muscle. The apparent molecular mass of a single polypeptide chain is similar to that of chicken gizzard filamin: 250-270 kDa. The protein interacts with antibodies against chicken gizzard filamin and induces F-actin gelation in a concentration-dependent manner. Immunofluorescent staining of cardiomyocytes and chicken heart sections with antifilamin antibody demonstrates two types of filamin localization: filamin was located on the sarcomere border in the periphery of the Z-disk; filamin was found in intercalated disks between cardiomyocytes.

MeSH terms

  • Animals
  • Chickens
  • Chromatography, DEAE-Cellulose
  • Contractile Proteins / isolation & purification*
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay
  • Filamins
  • Fluorescent Antibody Technique
  • Gizzard, Avian
  • Microfilament Proteins / isolation & purification*
  • Microscopy, Phase-Contrast
  • Myocardium / analysis*
  • Myocardium / ultrastructure
  • Myofibrils / analysis
  • Myofibrils / ultrastructure

Substances

  • Contractile Proteins
  • Filamins
  • Microfilament Proteins